Algorithms for Next-Generation Sequencing (Chapman & Hall/CRC Computational Biology Series)

A comprehensive guide to the computational frameworks used in modern genomics. The book introduces DNA sequencing technologies, categorizing them into three generations that range from early chemical degradation methods to advanced single-molecule real-time monitoring. It details the bioinformatics pipeline, beginning with raw data capture and moving through essential processes like read mapping, genome assembly, and variant calling. Additionally, the text explains standard NGS file formats—such as fastq for raw sequences and SAM/BAM for alignments—while highlighting the algorithmic data structures necessary for efficient data compression and analysis. Ultimately, the source serves as a technical foundation for understanding how massive biological datasets are translated into meaningful genomic and transcriptomic insights.

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