Ep. 10 Smart Chromatography: Purifying Full AAV Capsids from Empties

In this week's episode, we tackle another complex and critical challenge in gene therapy manufacturing: purifying recombinant Adeno-Associated Virus (AAV). Why is it so important to separate full capsids from empty ones—and how can we do it more effectively?

Join us as we revisit the fundamentals of chromatography and explore the cutting-edge role of mechanistic modeling in optimizing downstream purification. This episode bridges biology, engineering, and computation to reveal how smarter purification strategies lead to safer, more effective gene therapies.

Featured Article: Mechanistic Modeling of Empty-Full Separation in Recombinant Adeno-Associated Virus Production Using Anion-Exchange Membrane Chromatography (https://doi.org/10.1002/bit.28595)

Separation Techniques: Chromatography (https://doi.org/10.14744/nci.2016.32757)