The article details the development and application of a robotic nonlinear optical system designed for all-optical in situ histology of heterogeneous tissues. This innovative system combines iterative multiphoton microscopy with spatiotemporal line-focused laser micromachining to achieve submicrometer resolution 3D imaging and reconstruction of large-scale samples, such as the intact murine cranio...去小宇宙查看完整单集简介
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